highlighting growth rings in a photo

New Here ,
Aug 03, 2022 Aug 03, 2022

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Hi,

I have many hundreds of photos taken of fish otoliths. These are bony structures found inside a fishes head and when cross sectioned they show a series of increment lines which delineate annual periods of growth. For some species these annual increments are easily seen and counted (number of increments equals age of fish in years) but for others these lines are really faint. 

I am looking for the best approach to make these growth increments stand out when they are not very pronounced. I have attached a couple of pictures showing a good picture and a poor picture, along with markings to show where the increments occur. 

 

Any and all advice appreciated.

 

cheers


Andy

 

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Actions and scripting , Windows

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Adobe Community Professional ,
Aug 04, 2022 Aug 04, 2022

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You could use adjustment layers like Levels or Brightness and Contrast to bring out the subtle variations. The issue is they are very subtle. Here is an example:

kevinstohlmeyer_0-1659624264717.png

 

Kevin Stohlmeyer
Adobe Community Professional/Adobe Certified Instructor

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New Here ,
Aug 04, 2022 Aug 04, 2022

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Thanks Kevin,

I can see an improvement but as you say the changes are subtle. Otoliths are so variable in their readability and those from tropical fish even more so. I can see it will just require some patience and trial and error to get improvemens on a given image.

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Adobe Community Professional ,
Aug 05, 2022 Aug 05, 2022

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Last case effort would be to manually go in on a layer and paint the bands with a contrasting color.

Kevin Stohlmeyer
Adobe Community Professional/Adobe Certified Instructor

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Adobe Community Professional ,
Aug 09, 2022 Aug 09, 2022

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Some oversharpening might help somewhat. 

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Adobe Community Professional ,
Aug 05, 2022 Aug 05, 2022

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I would look at ImageJ and see if anyone on their forums has ideas. ImageJ has a huge scientific userbase and is well-suited for this kind of work.

https://imagej.net/

https://imagej.nih.gov/ij/

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