My GIF animation doesn't work when saved (Photoshop CS6)

[Jenny-pon]

Hello, 
I made an animation on Photoshop CS6, everything is working well in Photoshop but when I try to save it in GIF format ("save for Web"), it doesn't work anymore, even in the preview from the "Save for Web" window. 

I send here some screen captures (sorry, my Adobe Creative CS6 is in Japanese...). 

Can someone tell me what is wrong? 

Thank you!

Jenny-pon

[JJMack]

None of you layers look like they have transparency. If in the frame animation panels fly-out menu "New layer appear in all Frames" is checked. If you added a new top layer to the Layers stack all frames composite will be that top layer.  The Frame thumbnails in your screen capture are too small for my eyes to see.

[floramc]

What's that is not working? The transparent background? In that case it was already answered. Or is it the animation itself?