opacity option not available

[lissab57031009]

I'm working with close to 30 layers in a timeline animation. For some reason some of my layers have changed to 0% opacity. The opacity option on the layers window is grayed out for those layers. How can I change this?

[Myra Ferguson]

Can you post a screenshot of the full screen including your Layers panel?

[Mohammad.Harb]

Have you created any keyframes ?

have you used any Transition?

[lissab57031009]

Here's the screenshot. Yes, I had been playing with opacity but have removed all of those. One had been hiding in a group layer. I've removed that as well. Doing that allowed me to change back to 100% opacity on most of the layers but three remain grayed out, the first two and the last. Before I changed back the earlier layers the last file was at 100% but now it's reverted to 0%. Grrr. Is the file too large at 149.7 mb? Could it be corrupted?