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This is a weird issue and it has me baffled.
Using Premiere Pro 15.0.0 Build 41.
Windows 10 64bit - Intel i7-10700k w/32gb RAM.
When playing a subsequence, audio will only work in playback during the first bit of it. After that, audio will play bits while I scrub through the timeline or shuttle forward and back, but will not during normal playback.
I have a video here to demonstrate the problem: https://youtu.be/nOhAWqxcEa4
Any ideas on fixing this would be greatly appreciated.
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That is a strange one. First thing that comes to mind is are you using VFR Variable Frame Rate footage inside the nest? VFR footage sometimes works, but mostly it creates unique / unexpected problems.
You can use the free MediaInfo program to get info on your clips. Get the program from the link below and using the 'Tree' view post a screenshot of your clip info: https://mediaarea.net/en/MediaInfo/Download
You can use the free Shutter Encoder program to convert VFR footage to CFR (Constant) before adding it to PP.
https://www.shutterencoder.com/en/