Adobe Animate compiled file is not executed when run for the second time.

Hi, I have developed an animation game using Adobe Animate. When I first time compile the file, it was working properly. But then when I try to run it again, the file showing an error and is not executed. I tried to find the issue and resolve it. But I don't know why it is not executed since I has working perviously. So can you please provide me any help or suggestions regarding this issue.