TLF actually doesn't know where the characters are drawn - that's the job of the new text engine inside of Flash Player. So you can't get the information you want from any of the classes in the flashx.textLayout packages.
What you need is to drill down to the flash.text.engine classes. From the TLF class TextFlowLine you can get the corresponding flash.text.engine.TextLine, which has AtomBounds methods for determining where glyphs are placed inside the line. They're called Atoms because a single Atom may be a glyph representing one or more characters (for instance, an fi ligature is two characters but one atom)
var textLine:TextLine = flowline.getTextLine();
var firstAtomBounds:Rectangle = textLine.getAtomBounds(0);
var secondAtomBounds:Rectangle = textLine.getAtomBounds(1);
These rects will be relative to the TextLine's bounds.