Re-disable Javascript

Go to Menu - Preferences - JavaScript and tick off the first box, next to "Enable Acrobat JavaScript". This will stop JS from running anywhere in the application, and for all files.

@ron_6284 that is correct. After mistakenly choosing to always allow JavaScript for a specific PDF in Adobe Acrobat Reader, the setting is saved at the document level, which overrides your general preferences. This is why simply unchecking the "Enable Acrobat JavaScript" box in your global settings doesn't work for that specific file. To re-disable it, the simplest way to remove the "always allow" setting for that specific PDF is to create a new, clean copy of the file. You cannot edit the security settings within the free Acrobat Reader, so you'll essentially "re-print" the document to a new PDF. To do this, open the file, select the "Print" option, then choose "Microsoft Print to PDF" or "Adobe PDF" as your printer. Save the new file with a different name. This new PDF will not have the embedded JavaScript security setting from the original file, so it will now respect your general preference to keep JavaScript disabled.