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Alpha channels are greyscale, ranging from white to black with grey values in between.
Spot channels are a special type of alpha channel. As they are for print production, they use a subtractive colour model, where white = 0% and black = 100%.
Colour values are assigned to the spot channel via it's properties rather than by using a colour swatch or mixer values as would normally be the case for RGB/CMYK/Lab colour pixels.
There's a lot of room for error with spot channels if you're new to them.
What are you doing and where and how will the spot channel be used?
https://helpx.adobe.com/au/photoshop/using/channel-basics.html
https://helpx.adobe.com/au/photoshop/using/printing-spot-colors.html
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the spot color layer image will disappear,
No such thing (in Photoshop, so far); spot channels are Channels, not Layers.
Could you please post screenshots with the pertinent Panels (Toolbar, Layers, Channels, Options Bar, …) visible?