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VeiledDepths
Participant
August 19, 2025
Question

Picture rejection

  • August 19, 2025
  • 3 replies
  • 272 views

Hello there,

I have the problem that my microscopic pictures get rejected quite often, and I don’t really know why.
In microscopy, the organism looks sharp in parts, but the depth of field is usually very shallow, especially at higher magnifications — I can’t change that.
Also, floating particles or small specks are quite common, especially at higher magnifications. Could it be that these are interpreted as “dust” or “defects”?

 

Maybe someone can help ? 

3 replies

jacquelingphoto2017
Community Expert
jacquelingphoto2017Community Expert
Community Expert
August 21, 2025

Hi @VeiledDepths ,

The edge is not fluent. There is this missing edge here.

It's the same issue with the second photo. The edge looks crappy.

Best wishes

Jacquelin

 

 

Nancy OShea
Community Expert
Nancy OSheaCommunity Expert
Community Expert
August 19, 2025

Start by cropping in Photoshop or Lightroom, adjust levels and correct for white balance.  I had to do this type of work a lot for publication in scientific journals & text books. 

 

BEFORE:

 

AFTER:

Hope that helps.

 

 

 

Nancy O'Shea— Product User & Community Expert
V
VeiledDepthsAuthor
Participant
August 20, 2025

I'll try that too. Thank you! 

RALPH_L
Community Expert
RALPH_LCommunity Expert
Community Expert
August 19, 2025

I think sharpness is the issue. I looked at 200% and they are not crisp. I would try an Image shrapener like Topaz Photo AI.
See the difference?

    V
    VeiledDepthsAuthor
    Participant
    August 19, 2025

    Okay, yeah, i definitely see the difference. Thanks, i am going to try that 

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