After Effects roto render keeps re-enabling background

I have been using After Effects for a work project to rotoscope out some people from footage provided to me and I have done this process 6 times now but all of a sudden with this file, when I start to render and when viewing the file after rendering, the rotoscope effect is essentially disabled. The only signs that something is being rotoscoped is some small, transparent gaps in the final render. Images and export settings attached. I'm not sure what is going wrong here since I have done this many times, even a restart hasn't fixed it, help very much appreciated, thank you!