All the characters' recorded parts have stopped working

All the characters' recorded parts in my Character Animator project have stopped doing anything.

I have recorded parts for five characters - lip synch, dragger, face, motion library.

But after closing Character Animator and then opening the project again, all the recorded behaviours have stopped doing anything.

I can see all the recorded behaviours in the Timeline - but the characters don't do anything when I press Play. I can see the three puppets on the background in the Scene Panel, but when I press Play they remain motionless, as if I'd not recorded any behaviours for them.

Is someone able to advise me? - Unfortunately I can't upload the Ch project with all the data and media as it's 550MB.

Thanks in advance for any help, Greg