Instagram Audio Quality

If you want the best audio quality your audio tracks should be optimized in a program like Audition and saved as 40kHz 16 bit PCM WAV files. You should follow all audio standards. When you render your master using the Render Cue, or render for distribution using the Adobe Media Encoder, don't mess with the presets. They are all properly set up to industry standards. Fiddling with the audio and the video settings is asking for your streaming server to recompress your video with a sledgehammer. Everybody that streams video, runs it through their own compression system so they can build multiple versions to stream depending on the bandwidth. You have to follow their recommendations. I've never had any problems rendering audio from After Effects or Premiere Pro if I have properly prepared the source file and the final mix. This is something you can't eyeball, you have to know what you are doing and you have to know how to understand what the dials are telling you, and most of all, if you want a really good audio mix, you have to have a really good way to monitor the sound. Computer speakers are just barely adequate for mixing for computer speakers. 

I'm not clear what you are saying. It simply sounds like you're completely messing up the audio beforehand by overcranking the levels or individual frequency bands. Things like master levels and evening out the spectrum distribution do matter. Outside dedicated audio programs that support 24bit audio and such some stuff will simply be lost. That's just how it is. Normal audio players have no way of dealing with out-of-range amplitudes nor are they able to smooth out and reconstruct clipped samples. In addition, MP3/ MP4 will of course always be quantized to the respective bands used by their algorithms, so there is no point in using excessive settings to begin with, even more so since AE is terrible at resampling audio. Ideally, source audio and output audio settings must be identical so AE simply passes it through. Again, you almost definitely need to fix your audio files and master them suitably, not expect the encoders to handle out-of-spec audio correctly. Start by actually checking your audio waveform and at the very least bring it down to a common broadcast spec like -3 or -6 dB. That alone could greatly improve encoding results.

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