Unusual behaviour in transparency blend modes

[Mike_Red]

I am trying to develop a graphic design scheme wherein the overlapping area of circles on the text changes its colour. I used 'hue' in the transparency blend mode.

However, I'm seeing a peculiar behaviour by illustrator where it's only affecting the last vertical row of the circles even though all the circles have the same appearance. What is happening and how to solve it?

Here's the file. 

https://www.dropbox.com/scl/fi/brrkqbuqhlwrkk8zu4il7/PATTERNTEST.ai?rlkey=3adslb6w9p0eipe7vpixkc9xh&...

For some weird reason, illustrator files after getting attached gives this error and I am unable to share the file directly. 

[Ton Frederiks · Accepted solution]

Select one problem dot and look at the Appearance panel.

The ones that do not work have an additional blend mode: Darken.

[Mike_Red]

Thank you so much! That fixed it. 

[Mike_Red]

Also. Is it possible that the items are in separate layers and the blend mode still works? I have to keep them in the same layer to enable the blend mode's functionality. 

[Mike_Red · Accepted solution]

Ok. It works. My layer order was incorrect. Thanks!

[Ton Frederiks]

Good to hear that worked.

[Kurt Gold]

The circles left to the last vertical row have an additional blend mode (Darken) applied to their fills (see Appearance panel).

It should work as expected if you remove the Darken mode.