How do i turn of anti-aliasing in adobe animate

This tutorial is a little old but it could help.

https://www.youtube.com/watch?v=yr9iHJlbezo

Go to the library panel, select the bitmaps, right-click, properties, set compression to lossless/gif and uncheck smoothing. This method will work with the spritesheet generator.

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in the as3 publish settings for png and gif you can disable smoothing.  

i'm not sure what format your using for your sprite sheet, but probably png and i assume you're using an as3 project.

try it.  i haven't tested.