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I've got some guide layers in my comp, and on playback within After Effects, they aren't showing. I can see them when paused.
My understanding of guide layers is that they show within After Effects, including on preivew, but won't render upon export. At least…that's how they've worked in all my years of experience!
Anyway, I'm sure I've got some box checked that I don't know about that's responsible for this. Let me know what you guys know. Thanks!
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Try creating a new comp, adding a single shape, solid, footage, or text layer, making it a guide layer, and then running a preview. If the layer shows up, then the problem is probably in your composition. Guide layers in nested comps (pre-comps) don't show up in the main comp.
I suggest that you look for the problem by selecting the guide layers, pressing the 'u' key twice, and then looking for modified properties or effects.